Med. Weter. 68 (12), 717-721, 2012

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Bukowska D., Kempisty B., Piotrowska H., Antosik P., Jackowska M., Jaśkowski J.M., Bryja A., Nowicki M.
Selected aspects of in vitro fertilization in pigs
In the past few years increased progress in the in vitro maturation (IVM) and fertilization (IVF) of porcine oocytes has occurred. The recovery of oocytes with full developmental competition was possible in the case of improved in vitro culture (IVC) methods. However, there is still a large problem with polispermic embryos produced by IVF. The construction of culture media supplemented with hormones, amino acids and enzymes belongs to the most important factor in the decreasing of the polyspermy rate. These combinations lead to the decreased polyspermy rate and increased efficiency of fertilizations. The microsurgical injection of single spermatozoon into oocytes (ICSI) belongs to one of the most frequently used techniques. This method omits the natural selection of spermatozoa but may lead to the induction of several developmental defects in offspring. In this review the experimental results associated with in vitro techniques used in porcine reproductive biology have been presented.
Key words: fertilization, ICSI, fusion proteins, pigs