Medycyna Wet. 62 (2), 201-203, 2006
Adamska M.
Detecting Anaplasma phagocytophilum DNA in blood of roe deer and in ticks
The aim of the study was to establish the role of roe deer (Capreolus capreolus) as reservoirs of Anaplasma phagocytophilum and the role of Ixodes ricinus ticks as vectors of this pathogen in North–West Poland. Blood samples of 127 roe deer were collected between May and June 2004 and between September and December 2004. 51 of the 63 roe deer caught between May and June 2004 and 7 of the 64 roe deer caught between September and December 2004 were infested by I. ricinus ticks. 170 individuals of the common tick were collected from 45.7% of roe deer. PCR amplification of a fragment of the msp2 gene was used for detecting A. phagocytophilum DNA. Pathogen DNA was detected in 30 of the blood samples (23.6%) and in 10 ticks collected from the animals (5.9%). 28.6% of the roe deer caught between May and June 2004 and 18.75% of the roe deer caught between September and December 2004 were infected, but this difference was not significant. 5.3% of I. racinus ticks were infected by A. phagocytophilum. It is clear that C. capreolus is a significant reservoir of A. phagocytophilum in North–West Poland. In addition, I. ricinus is a significant vector in this area.
Keywords: Anaplasma phagocytophilum, roe deer, Ixodes ricinus