Medycyna Wet. 62 (2), 170-173, 2006
Łuszczewska-Sierakowska I.
Morphometrical analysis of neurons of the amygdaloideum’s nucleus corpus amygdaloideum baso-lateralis
The aim of the study was a quantitative examination (morphometrical analysis) of neurons during the maturation process of amygdaloideum baso-lateralis nuclei. The brains of domestic pigs of both sexes (obtained from the following periods: 7th-15th week of intrauterine life, newborn animals, one-month and oneyear old animals) were the examination material. The brains were removed and examined conventionally by microscope. The preparations were dyed according to Kltivera-Barrer’s method as well as the Nissel method. The histological preparations obtained in the above way were used to morphometrically analyze the neurons of the corpus amygdaloideum’s nucleus amygdaloideum baso-lateralis. Morphometrical examinations were carried out using the Nikon Eclipse E-600 microscope compressed with a JVC TK-1380-E camera and a computer using the MULTI-SCAN-BASE 08,98 morphometrical program. The following parameters were examined: the cross-section of nerve cells and the area of cell nuclei in µm2, the percentage of the nuclei-cellar rate, and the average diameter of nervous cell in µm, the volume of nervous cell in µm3, the number of neurons per 1 mm2, and the number of neurons per 1 mm3. Morphometrical observations revealed that cells forming the primary corpus of amygdaloideum in week 9 of fetal life are identical in shape and size. The size of the cell area in the 9th week of fetal life oscillates around 45 µm2. These cells, homogenous in the previous periods, undergo considerable differentiation during week 11 of fetal life. Mass nucleus structures appear from homogenous cells, including amygdaloideum baso-lateralis nuclei. The examined morphometrical parameters infer that neurons become morphologically mature at the end of fetal life.
Keywords: amygdala, brain, neurons, amygdaloideum nuclei