Medycyna Wet. 62 (2), 162-164, 2006
| Özdaş O. B., Taş M., Cirit U., Evecen M., Bacýnoğlu S., Demir K., Ak K., Ileri I. K. |
| Effect of different transport temperatures of cattle and sheep ovaries on in vitro maturation of oocytes |
| The aim of study is to determine the effects of different transport temperatures (4°C, 32°C) of sheep and cattle ovaries on the in vitro maturation of oocytes. Two experimental groups were formed. Sheep and cattle ovaries were put into saline solution at 32°C. The ovaries were transported to the laboratory, at the same temperature (Group I) or at 4°C following 10 minutes of incubation at room temperature (Group II) (n=6). Oocytes were collected from ovaries using the dissection method. Oocytes matured in their own group in 700 ml TCM-199 (supplemented with pyruvate, LH, FCS) for 23 h at a gas atmosphere of 5% CO2, 5% O2, 90% N2 and at 38.8°C. After maturation, oocytes were fixed in acetic acid-ethyl alcohol (1:3) for 48 hours. The stages of development up to MII, of the oocytes stained with aceto-orsein were then examined. The Chi-Square test was used for statistical analysis. While in the 4°C group, sheep oocytes reached 30.6% (MI), 15.3% (MII) and cattle oocytes reached 17.3% (MI), 46.8% (MII), in the 32°C group these percentages were respectively 38.3%, 33.1% in sheep and 19.3%, 55.4% in cattle. While oocytes obtained from sheep ovaries transported at 32°C reached the MII stage at a higher rate compared to those at 4°C (P<0.001), no statistically significant difference was observed between maturation to the MII stage of oocytes obtained from cattle ovaries transported at 4°C and 32°C. As a result of this study, it was established that cattle ovaries could be transported both at +4 C, +32°C and that there was no difference in oocyte maturation. |
| Keywords: Keywords: Ovary transport, in vitro maturation, bovine, sheep |