Medycyna Wet. 62 (7), 824-826, 2006
Radzki R. P., Bieńko M., Kapica M., Puzio I., Dobrowolski P., Filip R.
Effect of β2-adrenergic receptor agonist clenbuterol on the mineralization of tibiae in rats with established osteopenia
The purpose of the study was to determine the effect β2 -adrenergic receptor agonist.clenbuterol on tibiae mineralization of female rats with established osteopenia. The experiments were conducted on 30 female 3-months-of-age Wistar rats with an initial body weight of about 250 g. The sham-operation . SHO (n=10) and bilateral ovariectomy . OVX (n=20) were performed. After 60 days of osteopenia induction the ovariectomized rats were divided into a group fed a standard diet (n=10) and a group that received a diet supplied with clenbuterol at a dose of 5 mg/kg. After 14 days of the experiment the tibiae was isolated and tested using a DEXA densitometer, peripheral quantitative computer tomography (pQCT) and three-point bending test. The obtained results proved that a 14 day period of clenbuterol treatment significantly increased mechanical properties content and mineral density, both planar (BMD) and volumetric (vTotBMD) of the tibiae of ovariectomized rats.
Keywords: clenbuterol, adrenoreceptors, rats, bone biomechanics