Medycyna Wet. 62 (6), 717-719, 2006
| Witek B., Graniczka A., Ochwanowska E., Kołątaj A. |
| Changes of the lysosomal enzyme activity in the liver and kidney of mice after glucagon injections |
| Changes in the activity of β-glucuronidase, N-acetyl- β -glucosaminidase, cathepsin D and cathepsin L in the liver and kidney of mice, injected intraperitonealy with 15 µg/kg b.w. of glucagon were investigated. The experiment was carried out on 24 8-week-old mice, whose parents were chosen from random match. The homogenates of the liver and kidney were subjected to differentiate centrifuging, and in the lysosomal fractions of the liver and kidney the activities of β -glucuronidase [β -GlcUr], N-acetyl- β -glucosaminidase [NAG], cathepsin D [Cath. D], and cathepsin L [Cath. L] were estimated. Injection of glucagon caused a decrease in the activity of β -glucuronidase in the liver and kidney, cathepsin L in the liver, N-acetyl-b-glucosaminidase in the kidney, and an increase cathepsin D and cathepsin L in the kidney of mice. The results suggest that exogenous glucagon had a significant influence on the activity investigated lysosomal enzymes. The range of the reaction remained in a relationship with the kind of the organ and type of enzyme. |
| Keywords: lysosomal enzymes, glucagon, mice |