Medycyna Wet. 62 (9), 1033-1034, 2006
Natonek-Wiśœniewska M., Słota E., Rejduch B.
Methods of detecting animal components in feedstuff
Epizootiological studies have shown that meat-and-bone meal of ovine or bovine origin added to feed concentrates are infectious agents in bovine spongiform encephalopathy (BSE). In view of the ban on the use of meat-and-bone meal in animal feed in European Union countries, the aim of the study was to elaborate methods for identifying chicken and sheep DNA in compound feeds. A duplex PCR reaction (simultaneous amplification of chicken and sheep DNA) was used to identify mtDNA specific to chickens and sheep. The reaction products were subjected to electrophoresis in 4% agarose gel in the presence of X 174DNA/HaeIII marker. The results obtained enabled the identification of DNA specific to both chickens and sheep.
Keywords: BSE, MBM, PCR