Medycyna Wet. 63 (1), 80-83, 2007
Turowska A., Chmielewska A., Tucholska A., Bańbura M. W.
Influence of equine herpesvirus type 1 (EHV-1) infection on actin cytoskeleton
The aim of the study was to investigate the influence of equine herpesvirus type 1 (EHV-1) infection on actin cytoskeleton in ED (equine dermal). Cells in vitro.ED cells were infected with a strain of Jan-E of EHV-1, fixed and stained for the presence of actin and virus antigen. Results were evaluated by confocal microscopy. The assembly of the actin cytoskeleton was heavily disturbed. In order to affirm whether changes in cytoskeleton of EHV-1 infected cells depend on the type of cells, we infected Vero cell culture with 2 different standard strains of EHV-1 – Rac-H, AIV – and Jan-E isolated from an aborted fetus. Unfortunately, the infection of Vero cells with the strain Jan-E of EHV-1 failed because this strain was not adapted to the heterogeneous cell line. Only strain Rac-H and AIV can replicate in Vero cells, which was determined through the application of PCR.
Keywords: actin, cytoskeleton, EHV-1