Medycyna Wet. 63 (6), 655-657, 2007
JóźŸwik A., Jakubowski T., Kaba J., Jurkowski W., Witkowski L., Nowicki M., Frymus T.
Evaluation of the agreement of ELISA and complement fixation test in the diagnostics of Q fever in cattle
Q fever is a worldwide zoonosis that is manifested as a reproductive failure in animals and by polymorphic, nonspecific symptoms in humans. The infection may be acquired through the respiratory or alimentary route or an arthropod bite. The diagnosis of Q fever relies mainly upon serology: indirect immunofluorescence assays (IFA), complement fixation (CF) tests or enzyme-linked immunosorbent assays (ELISA) are used to detect antibodies against Coxiella burnetii in sera of infected animals. The aim of our study was to evaluate the agreement of the commercially available ELISA kit and CF test using statistical methods (Kappa value). We used serum samples that were collected from 122 dairy cows from one herd in central Poland. The general health status of the herd was good, and the animals were clinically normal. Our results showed a low agreement (Kappa value = 0.376) between the commercially available ELISA and CF test.
Keywords: Q fever, CF test, Kappa value