Medycyna Wet. 64 (9), 1157-1160, 2008
| Stenzel T., Tykałowski B., Andrzejewski M., Koncicki A. |
| The influence of methisoprinol applied in ovo on the percentage of CD3+, CD4+ and CD8+ T-lymphocyte subpopulations in turkeys. |
| The aim of the research was to determine the influence of a synthetic immunomodulator – methisoprinol applied in ovo as a 10% solution in doses of 5 mg per egg (group I) and 20 mg of active substance per egg (group II) on the 26th day of incubation - on T-lymphocyte subpopulations in the blood and spleen of 5-day-old turkeys hatched from the treated eggs. The control group (group III) were turkeys hatched from eggs in standard hatchery conditions (without in ovo injections). The percentage of T-lymphocyte subpopulations was determined by flow cytometry using specific monoclonal anti-T CD3+, CD4+ and CD8+antibodies and an EPICS XL apparatus. It was demonstrated that methisoprinol applied in ovo in doses of 5 mg per egg stimulated non-specific mechanisms of humoral immunity in 5-day-old turkey poults hatched from the treated eggs, which resulted in a higher percentage of CD3+ and CD4+ T-lymphocytes in their blood and spleen. Methisoprinol applied in ovo in doses of 20 mg per egg stimulated mainly non-specific mechanisms of cellular immunity in 5-day-old turkey poults hatched from the treated eggs, as evidenced by a higher percentage of CD8+ T-lymphocytes in the spleen. |
| Key words: CD3+, CD4+ and CD8+ T-limphocytes, turkeys, methisoprinol. |