Medycyna Wet. 65 (1), 55-60, 2009
Czeczot H., Ścibior-Bentkowska D., Skrzycki M., Podsiad M., Karlik W., Bąkała A., Grono D., Wiechetek M.
Effect of cadmium on the activity of antioxidant enzymes in isolated rat hepatocytes.
The aim of the study was to evaluate the effect of cadmium on the antioxidant enzyme activity in rat hepatocytes. The experiments were conducted on isolated rat hepatocytes, which were incubated for two hours in modified Williams’ E medium (MWE) with 25, 50, and 200 μM cadmium chloride (CdCl2). Hepatocytes incubated in MWE medium without cadmium chloride were used as a control. The activity of antioxidant enzymes - superoxide dismutases (SOD1 and SOD2), catalase (CAT), total glutathione peroxidase (tot. GSHPx), selenium-dependent glutathione peroxidase (Se-GSHPx), glutathione S-transferase (GST) and glutathione reductase (GSHR) - and the values of the studied oxidative stress markers – the concentration of tiobarbituric-acid-reacting substances (TBARS) and reduced glutathione (GSH) - indicate that cadmium induces oxidative stress in rat hepatocytes, which impairs antioxidative mechanisms.
Keywords: cadmium, isolated rat hepatocytes, oxidative stress, antioxidant enzymes