Medycyna Wet. 65 (7), 475-479, 2009
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| Adaszek Ł., Winiarczyk S., Jankowski Ł., Maj J., Ziętek J. |
| Molecular analysis of CDV hemagglutinin gene isolated from clinical cases of distemper in foxes and dogs. |
| The aim of the study was to to detect and characterize the canine distemper virus isolated from 4 foxes and 6 dogs, naturally infected in Poland, as well as from two vaccines. A 200-bp fragment of the CDV H gene was amplified and sequenced. Sequencing of the PCR products from the isolates led to the identification of 4 groups. The most numerous group No. 1 was represented by virus strains isolated from 4 foxes and 4 dogs. In group No. 2 there were two sequences of CDV isolated from two dogs. These sequences varied from group 1 by transitions in positions 133 and 135. The isolates from groups 1 and 2 showed significant differences of nucleotide as well as aminoacid sequences in comparison with the isolates obtained from vaccines and with various CDV sequences obtained from GenBank. The results may indicate the appearance of new CDV variants in eastern Poland |
| Keywords: CDV, RT-PCR, N-gen, sequencing. |