Medycyna Wet. 65 (11), 789-791, 2009
| Karpińska E., Żbikowski A., Szeleszczuk P., Kosowska G., Malicka E. |
| Infectious laryngotracheitis in a Crested Polish flock- case report |
| Infectious laryngotracheitis (ILT) is a disease that causes high death losses in susceptible poultry. In autumn 2007, a number of live and dead chickens from a Crested Polish breeding of 150 birds were submitted to the Institute of Poultry Diseases. Clinically, the affected birds showed severe symptoms of respiratory system irritation (gasping, difficulties in breathing, wheezing), whereas at necropsy, haemorrhagic inflammation of the mucosa of the larynx and of the upper part of the trachea was noted. Pathologically changed segments of the respiratory system and swabs from the buccal cavity were taken for laboratory examination. Histopathological examination of cell nuclei in the affected respiratory tract revealed Seifried intranuclear inclusion bodies that are characteristic of ILT. Virological examination of the chorionic-allantoic membrane in 11-day chicken embryos showed changes typical for ILT virus replication in the first passage. The presence of viral DNA was detected by the PCR method in all samples from swabs, chorionic-allantoic membrane, and segments of the trachea. The disease outbreak was caused by the introduction of vaccinated birds into a non-vaccinated flock. |
| Keywords: ILT, Crested Polish, histopathology, PCR. |