Medycyna Wet. 66 (2), 118-120, 2010

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Paprocka G.
Detection of swine vesicular disease virus (SVDV) in biological material
Swine vesicular disease virus (SVDV) is a member of the genus Enterovirus in the family Picornaviridae. This virus appears to have evolved from human coxsackievirus B5. Pigs infected with this virus show almost identical clinical signs to foot-and-mouth disease in pigs. Vesicular diseases must be differentiated with laboratory tests. The purpose of the study was to apply the isolation test in cell culture and RT-PCR assay for the detection of swine vesicular disease virus in the epithelial and fecal samples. Out of a total of 11 examined samples, 10 were found positive using these methods. The antigen ELISA was used for the confirmation of specificity of isolation assay. Primary piglet kidney cells and certain IB-RS-2 cells were a sensitive cell culture system for the detection of swine vesicular disease virus, whereas secondary lamb kidney cells not.