Medycyna Wet. 66 (12), 847-851, 2010
Małaczewska J.
In vitro effect of silver nanoparticles on the viability and proliferative response of mice peripheral blood mononuclear cells and splenocytes
Silver nanoparticles (SNP) have been recently one of the most widely utilized nanomaterials, mostly because of their antimicrobial activity. For some time there has been a great interest in SNP of unconventional medicine, which recommends their use not only as an antimicrobial, but also as an immunostimulant. However, little is known about SNP’s impact on immunocompetent cells in vitro. The aim of a present study was to investigate the influence of the colloidal nano-silver solution on the viability and proliferative response of mice peripheral blood mononuclear leukocytes and splenocytes in vitro. After isolation cells were cultured in complete RPMI – 1640 medium containing 0 (control), 20, 10, 5, 2, 1, 0.5, 0.2, 0.1 and 0.05 ppm of SNP, for 24 and 48 h to investigate SNP’s impact on cell viability, and for 72 h to evaluate their effect on the proliferative response of cells. Both parameters were assessed using MTT assay. Obtained results suggest that SNP have significant influence on the both investigated parameters. High doses of SNP significantly decreased (p < 0.01) the viability (concentrations of 5-20 ppm for splenocytes and 2-20 ppm for leukocytes, respectively) and proliferative response of cells (splenocytes: 5-20 ppm, leukocytes: 10-20 ppm) whereas low SNP’s doses slightly increased (p > 0.05) the viability of cells (splenocytes: 1 ppm, leukocytes: 0.2 ppm) and significantly increased stimulation index of both cell types induced by mitogens (splenocytes: 0.2-0.5 ppm, p < 0.05; leukocytes: 0.1-0.5 ppm, p < 0.01). These experimental data constitute an encouragement for further investigations concerning the possibility of therapeutic use of SNP’s low doses.
Keywords: silver nanoparticles, splenocytes and leukocytes viability, proliferative response