Medycyna Wet. 67 (3), 194-197, 2011
| Hoszowski A., Lalak A., Zając M., Samcik I., Skarżyńska M., Wnuk D., Wasyl D. |
| Relationships of rough Salmonella strains with representatives of some serovars found in animals |
| The objective of this study was to provide an epidemiological characterisation of rough Salmonella strains and to evaluate their genetic relationship with some representatives of serovars noted in animals. Genome macrorestriction by means of pulsed field gel electrophoresis of 57 rough strains revealed 8 groups showing high genetic similarity to the profiles observed in Salmonella Enteritidis, Salmonella Mbandaka, Salmonella Typhimurium, Salmonella Goldcoast, Salmonella Infantis, Salmonella Hadar, Salmonella Oranienburg and Salmonella Derby. Some of them showed profiles indistinguishable from the representatives of the above-mentioned serovars. These included also the most numerous group of “Salmonella Enteritidis-like” strains. This proves epidemiologic relationships within this group as well as with the reference strains, representing serovars found in animals. It was concluded that the molecular typing of rough strains provides additional epidemiological information and could sufficiently support routine Salmonella diagnostics, including autoaglutinating isolates. |
| Keywords PFGE, Salmonella diagnostics, autoaglutination, rough strains |