Medycyna Wet. 67 (8), 527-530, 2011
Kempisty B., Zawierucha P., Nowicki M.
Using of DNA and RNA microarrays in order to identify oncogenesis markers in mammals
In recent years an increased number of diagnosed cancers in mammals have been noted, mainly in dogs, and has lead to intensive research based on the causes and course of cancers in animals. The development of molecular biological techniques, statistics and bioinformatics has made possible the development of new diagnostic methods. The DNA microarrays is a technique that is prepared for the identification of new mutations within the scale of the whole genome as well as in the determination of the frequency of single nucleotide polymorphisms (SNP). A consequence of the development of this method is the creation of RNA microarrays based on transcriptomics technology. Using both of these methods in the scale of whole genome and/or transcriptome leads to describing the causes of oncogenesis induction as well as suggests more efficient methods of therapeutic treatments. In this article the methodological characteristics and possible practical application of DNA and RNA microarrays in research on mammalian oncogenesis has been presented.
Key words: Microarrays, SNP, Gene expression profiling, transcriptomics.