Med. Weter. 69 (3), 187-190, 2013

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Snarska A., Krystkiewicz W., Żarczyńska K., Rękawek W., Sobiech P., Radwińska J.
Evaluation of bone marrow in Holstein-Friesian cattle infected with the BVDV virus.
Bone marrow examinations were performed in a group of 10 Holstein-Friesian (HF) cows aged 2 years, which had been diagnosed with BVD-MD infection in a PCR screening test. Prior to bone marrow sampling, peripheral blood samples were collected for hematological analysis. Bone marrow was sampled from the third and fourth ribs in the sternal region, and it was placed in test tubes containing no anticoagulant. Smears were stained by the MGG method. The aim of this study was to demonstrate changes in the process of hematopoiesis in animals with clinical signs of BVD / MD The results of the analysis revealed changes in erythrocyte, macrocyte-macrophage, lymphoid and granulocytic cell lines. There was a decrease in the number of red blood cells in the peripheral blood and polychromatic and ortochromatic erythroblasts in the bone marrow. A decrease in leukocyte counts below reference values resulted mainly from a decrease in the number of neutrophilic metamyelocytes, eosinophilic myelocytes and banded eosinophils. Moreover, a decrease in the number of megakaryocytes was observed in the megakariocytic system. BVDV infection causes changes in the activity of the hematopoietic system.
Key words: cattle, bone marrow, BVD/MD.