Med. Weter. 69 (3), 145-149, 2013

full text

pdf
Adaszek Ł., Jankowska M., Wyłupek D., Winiarczyk S
Usefulness of LAMP in the fast diagnosis of babesiosis in animals
Nucleic acid-based methods offer a variety of tools for the detection of parasites. This field of veterinary and medical sciences is rapidly evolving. Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid method whereby DNA is amplified with high specificity, efficiency, and rapidity under isothermal conditions using a set of four specifically designed primers. In this paper we present the usefulness of LAMP method in the diagnosis of babesiosis in various animal species. Babesia species-specific LAMP assay may have potential clinical application in the detection and differentiation of Babesia species, particularly in countries in which babesiosis is endemic. It can be assumed that in the near future this technique will be a routinely used diagnostic test in veterinary practices.
Key words: Babesia, molecular diagnostic, LAMP