Med. Weter. 69 (6), 358-362, 2013

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Selwet M., Galbas M., Porzucek F., Koczorowski M., Pociejowska M.
Effects of the method of alfalfa ensiling on the content of biogenic amines and numbers of some strains of Lactobacillus spp.
The investigations involved determining the influence of the way of ensiling of alfalfa on the content of some selected biogenic amines in silages, changes in the content of dry matter, crude protein, lactic and acetic acids as well as of ammonia. The performed microbiological analysis entailed determining total counts and the frequency of occurrence of: Lactobacillus spp. (L. plantarum, L. buchneri, L. brevis), as well as Enterobacteriaceae bacteria mainly responsible for the level of amine concentrations. Experimental silages were prepared from fresh and wilted Lucerne, also using a chemical additive. The highest biogenic amine totals and the highest counts of Lactobacillus spp. and Enterobacteriaceae bacteria were recorded in silages prepared from wilted alfalfa. The applied chemical additive reduced the level of amines in silages and Enterobacteriaceae counts.
Key words: biogenic amines, alfalfa, silage, Lactobacillus spp.