Med. Weter. 69 (11), 692-695, 2013

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Pękala A., Paździor E., Kozińska A., Dragan M., Suszyński M., Niemczuk W., Reichert M.
Studies of the occurrence of the epizootic ulcerative syndrome (EUS) in ornamental fish in Poland
The aim of this study was to diagnose the presence of the fungus Aphanomyces invadans, the etiological agent of the epizootic ulcerative syndrome (EUS), in ornamental fish in Poland. Thirteen species of ornamental fish from Polish culture, as well as imported from Asia, Africa and South America, exhibiting haemorrhages, ulcers, and skin lesions or mortality, were examined during three years of studies. Samples from skin lesions, muscles and internal organs (kidney, liver) were collected. Each sample was inoculated on GP medium to enable mycelia to grow, as well as diagnosed directly by the PCR method. Each specimen of mycelia grown on the GP medium was examined morphologically and by the PCR technique. In total, 481 muscle samples, 497 skin samples, 133 kidney samples, and 30 liver samples were examined. The fungus A. invadans was not observed in cultures on the GP medium. The tests of the samples collected from the tissues and organs showing pathological signs, performed by the PCR method, were also negative. One should bear in mind that the fish examined constituted a very small percentage of the total population of ornamental fish bred in Poland and imported into our country. These examinations, performed for the first time in Poland, made it possible to evaluate the epizootic situation concerning the EUS in our country.
Key words: Aphanomyces invadans, EUS, ornamental fish