Med. Weter. 69 (11), 687-691, 2013

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Kwit K., Pomorska-Mól M., Markowska-Daniel I.
course of pregnancy in gilts after intranasal infection with the H1N2 subtype of swine influenza virus during the first month of pregnancy
Pregnant gilts (in the first month of pregnancy) were infected intranasally with SIV H1N2. Clinical signs, presence of SIV RNA in nasal swabs, haemagglutination titer, hematological parameters, concentrations of interleukin (IL-10, IFN) and acute phase proteins (APP) (CRP, Hp), as well as production parameters, were evaluated. The concentrations of APP and cytokines were analyzed with the use of ELISA assays. Antibodies against SIV were detected with the haemagglutination inhibition assay, and the presence of RNA with RT-PCR. No clinical sings typical of swine influenza were found in the infected gilts. The gilts did not show any signs of pregnancy pathology, and no abortions were observed. No significant differences were found between the infected and control gilts with regard to the number of piglets born, hematological parameters, or the concentrations of APP and cytokines. The presence of specific antibodies against SIV was confirmed in the infected gilts starting from 14 dpi. The presence of SIV RNA in nasal swabs collected from the gilts was confirmed within 4 dpi. No SIV RNA were found in samples taken from newborn piglets.
Key words: swine, swine influenza, H1N2, reproduction