Med. Weter. 70 (8), 472-478, 2014

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Kunowska-Slósarz M., Baumgart U., Boruta A., Gołębiewski M.
In vitro maturation of the oocytes of domestic dog (Canis lupus familiaris)
The efficiency of the in vitro maturation of canine oocytes is still relatively small. The periovulation period in dogs is different from that in other animal species. This study presents the results of research on the use of various media and substances, such as serum and serum albumin, hormones, energy substrates, antioxidants, antibiotics, antimycotics, and other compounds supporting the maturation of oocytes in vitro. It also describes the methods of culturing oocytes (incubation in a drop, incubation with somatic cells, incubation in the isolated oviduct and in isolated follicles). If the research on conditions simulating the oviduct environment is continued, it may become possible to obtain a higher number of oocytes capable of fertilization than at the present time.
Key words: dog, in vitro maturation, oocytes