Med. Weter. 71 (5), 286-291, 2015

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Robak P., Ożgo M., Herosimczyk A., Lepczyński A., Pyć A., Skrzypczak W.F.
Kidney proteomic profiling of African catfish
The purpose of this study was to create a two-dimensional (2-D) profile of kidney proteins characteristic of African catfish (Clarias gariepinus). We prepared proteins from kidney tissues of healthy 8-month-old African catfish. Samples were subjected to separation using high resolution two-dimensional electrophoresis (2-DE) and stained with colloidal Coomassie Brilliant Blue G-250. Among the 96 protein spots detected on each 2-DE gel, 9 different proteins were identified by means of a matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer. These proteins belong to the following groups: structural (actin and tubulin), involved in fat transport (fatty-acid binding protein), involved in cell cycle regulation (ubiquitin-protein ligase), responsible for cell metabolism (ATP synthase, hypoxanthine-guanine phosphoribosyltransferase, enolase) and molecular chaperone (heat shock protein 70kDa). The presented in the current study two-dimensional map of kidney proteins may provide a basis for further refinements in the proteomic analysis of fish, including African catfish. The final result of comprehensive proteomic analysis may be a better understanding of kidney physiolo-gy and pathology, as well as identification of health and disease-related biomarkers.
Key words: Clarias gariepinus, kidney, proteomics