Med. Weter. 71 (7), 453-457, 2015
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| Mikiewicz M., Otrocka-Domagała I., Gesek M., Paździor-Czapula K., Rotkiewicz T. |
| Immunohistochemical and histopathological evaluation of malignant pheochromocytoma: a case study |
| Malignant pheochromocytoma is an uncommon neoplasm in dogs. This study evaluated the histopathological and immunohistochemical profile of malignant pheochromocytoma. The report presents a case of bilateral adrenal gland tumor in a 6-year-old female golden retriever, diagnosed during necropsy. The necropsy also showed changes in other organs, i.e. heart (left ventricular hypertrophic cardiomyopathy, right ventricular dilated cardiomyopathy and infarction foci), lungs (multifocal alveolar emphysema and atelectasis). Metastases were not present. A histological examination showed that neoplastic cells were round, oval, polygonal and spindle with a high degree of anisocytosis and anisokaryosis. The nuclei were small, round, oval and some were divided. The nucleoli were faintly visible, and numbered from 2 to 3. The number of mitotic figures per 400 high power fields ranged from 0 to 2. Immunohistochemical staining using an antibody panel revealed that the tumor cells were strongly positive for chromogranin A, S100 protein and neuron specific enolase, but were negative for Ki67. PCNA expression was observed in some neoplastic cells, especially in those located close to the connective tissue stroma. Malignant pheochromocytoma was diagnosed on the basis of a immunohistochemical examination and tumor morphology. |
| Key words: adrenal gland tumor, S100 protein, chromogranin A (CGA), neuron specific enolase (NSE), proliferating cell nuclear antigen (PCNA), Ki67 |