Med. Weter. 72 (1), 46-52, 2016
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| Kempisty B., Zaorska K., Bukowska D., Nowak M., Wojtanowicz-Markiewicz K., Porowski S., Ociepa E., Antosik P., Brüssow K.-P., Bruska M., Nowicki M., Zabel M. |
| Analysis of mitogen-activated protein kinase (MAP2K2) gene polymorphisms in relation to the occurrence of adenocarcinoma during aging in dogs |
| In the most recent years adenocarcinoma has been found to be the most common cancer that occurs in both humans and animals. It is well recognized that the induction of carcinogenesis and/or cancer growth and development is often associated with aging. Moreover, the molecular basis of carcinogenesis involves disturbances in expression of genes or mutation in genes that play a crucial role in regulating cell division cycle and inter- or intracellular signaling pathways. MAP2K2 belongs to the large family of kinases that are described as “checkpoints” of cell division and signaling, and therefore may be involved in carcinogenesis. In this study, blood samples were obtained from 22 female dogs diagnosed with mammary tumors. Moreover, blood samples were obtained from geriatric (> 5 to 10 years old; n = 15), mature adult (> 2 to 5 years old; n = 10) and young (from 1 to 2 years old; n = 11) individuals. 36 bitches diagnosed because of other reasons served as controls. After Sanger sequencing analysis we found 17 single nucleotide variations, of which 3 were situated in exons (exon 2, 3 and 11), 2 other in 5’UTR non-coding region and the remaining 12 in splice regions of introns. Some of the polymorphisms, such as g.C-81T, could have higher probability of being involved in tumor development, also in correlation with aging. Furthermore, both variants c.A384G and g.T9144C were associated with strong risk factors of tumor occurrence and aging. In conclusion, it may be suggested that some of the MAP2K2 gene polymorphisms may be recognized as markers for occurrence of adenocarcinoma in dogs. This also showed that possible disruption in expression of MAP2K2 protein kinase may lead to the induction of carcinogenesis, since it plays a crucial role in regulating the cell division cycle and cell signaling. |
| Key words: mutation, cancer, Canis familiaris |