Med. Weter. 2020, 76 (11), 631-637
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| VELI GULYAZ, BANU BAYRI OZBILGE, NESLIHAN TASCENE, SUKRAN YILMAZ, YASEMIN GULTEKIN, GONCA OZTAP, MUSTAFA HASOKSUZ |
| Toxicity level of the tulathromycin on BHK cell culture and the effect on infective titers of foot and mouth disease viruses |
| The aim of the study was to evaluate the cytotoxicity of tulathromycin (macrolide antibiotic) on BHK-21 An30 cells and to determine the effect on FMD (Serotypes; A/TUR/11, O/TUR/07, Asia-1/TUR/15) virus propagation in vitro. In the result of MTT tests and cell culture studies, the non-toxic upper concentration of the tulathromycin limit for BHK-21 An30 cell culture was determined as 30 µg/ml in terms of cell morphologies and numbers. The mean FMD 146S virus particle values produced in BHK-21 An30 cell cultures containing 30 µg/ml of tulathromycin were found to be 0.47, 0.57 and 0.25 µg/ml; the mean infective titer determined as 107.03, 106.23 and 107.40 pfu/ml for A/TUR/11, O/TUR/07 and Asia-1/TUR/15, respectively. In the FMD virus cultures produced with medium containing penicillin-streptomycin, the mean values of 146S virus particles were determined to be A/TUR/11, O/TUR/07 and the Asia-1/TUR/15 serotypes were 0.50, 0.55, and 0.32 µg/ml, respectively, while the mean infective titers were 107.46, 106.62 and 107.73 pfu/ml, respectively. As a result, it was concluded that tulathromycin can be used as an antibiotic up to 30 µg/ml in a BHK-21 An30 cell culture and in the production media of the FMD virus A, O, and Asia-1 serotypes. |
| Keywords: tulathromycin, BHK cell culture, FMDV, antibiotics, contamination |