Med. Weter. 79 (10), 520-524, 2023

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Genetic characterization and virus isolation of porcine reproductive and respiratory syndrome virus
Porcine reproductive and respiratory syndrome virus (PRRSV) is an acute, febrile, highly contagious infectious disease characterized by reproductive dysfunction and respiratory failure. Its high morbidity and mortality have caused significant economic losses to the swine industry. With the continuous development of PRRSV, its genetic variation is also increasing, which causes great difficulties for the clinical prevention and control of infections casued by the virus. Monitoring the genetic variation of the virus and vaccine immunity at any time is the current research focus. In this study, patients suspected of having PRRSV were sampled, and the virus was successfully isolated. The genomic variation characteristics of the new isolate of HLJ03 were determined, especially the molecular characteristics of GP5 and NSP2, and the main focus was placed on the analysis of genetic variation. The results provide important information for further investigation of the molecular prevalence of porcine PRRSV and create a basis for the prevention and control of porcine PRRSV infections.
Keywords: Porcine reproductive and respiratory syndrome virus; virus isolation; genetic analysis