Med. Weter. 73 (5), 284-288, 2017

full text

Zafer Yazici, Semra Gumusova, Cuneyt Tamer, Abdurrahman A. Cagirgan, Ayhan Akman, Emre Ozan, Hamza Kadi, Hasan S. Palanci, and Harun Albayrak
Molecular identification and phylogenetic analysis of non-cytopathic bovine viral diarrhea virus isolate obtained from a local respiratory outbreak in Northern Turkey
In February 2016, a local respiratory disease outbreak with two fatalities was reported in Samsun, Turkey. A non-cytopathic bovine viral diarrhea virus (ncp-BVDV) was identified from the organ and leucocyte samples of dead juvenile heifers using RT-PCR with specific primers for the NS2/3 gene coding region. The NS2/3 gene of BVDV was sequenced and compared with other published sequences of BVDV. The sequences of our isolate which was named as Samsun TR, had 81–83% nucleotite (nt) identity for BVDV-1. Phylogenetic analysis revealed that Samsun-TR was closely related to LC089875 (Japan), AF526381 (China) and also shared 83% nucleotide(nt) identity with them. The NS2/3 gene sequence of Samsun-TR was deposited in the GenBank database with the accession number of KX428495.
Key words: BVDV, RT-PCR, phylogenetic analysis, nucleotide sequence, ELISA