Medycyna Wet. 62 (2), 204-206, 2006
| Krumrych W. |
| Influence of selected stimulators on chemiluminescence of peripheral blood neutrophils in horses |
| The purpose of the study was to assess the condition of peripheral blood neutrophiles in horses through using the luminal-enhanced chemiluminescence (CL) method. The study population consisted of 33 clinically healthy horses of both sexes (aged 315 years). The chemiluminescence of non-stimulated neutrophiles was measured as well as of those stimulated by a receptor stimulus - myosin (Z), opsonic zymosan (OZ) and formyl-Met-Leu-Phe (fMLP) and an extrareceptor stimulus - phorbol myristate acete (PMA). The results of the study demonstrated that the chemiluminescence of neutrophiles stimulated by Z and OZ was considerably higher in comparison to the values of CL stimulated by fMLP and PMA. The use of stimulators can trigger a "respiratory burst" and may be helpful in assessing the phagocyte activity of equine neutrophiles. |
| Keywords: chemiluminescence, neutrophiles, horses |