Medycyna Wet. 64 (1), 59-63, 2008
| Dümen E., Ünsal Baca A., Dümen E. |
| Comparative detection of Listeria monocytogenes in raw milk by microbiological method and PCR |
| In this study, 150 raw milk samples from 15 different farms were collected in the city of Istanbul, and the raw milk samples were analyzed for Listeria monocytogenes by both conventional microbiological methods and PCR. According to the authors' results, 11 of 150 raw milk samples (7.33%) were detected to be Listeria monocytogenes positive by the both techniques. The results of the conventional microbiological analysis and the PCR procedures were parallel to each other. PCR must be used more frequently in food microbiology science, to protect public health and to prevent economic losses, because of its high rate of accurate results, time advantages, sensitivity and ability of producing desired DNA counts of the target microorganism. |
| Keywords: Listeria monocytogenes, PCR, milk |