Medycyna Wet. 65 (2), 99-101, 2009
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| Król K., Samorek-Salamonowicz E., Kozdruń W., Woźniakowski G. |
| Comparison of different methods for the detection of Mareks disease virus |
| The aim of this study was the comparison of accordance, sensitivity, and specificity of the radial agar gel immunodiffusion test (RID) and PCR for the detection of Mareks disease virus (MDV) infections. Feather tips, liver, and spleen sections collected during necropsy from 256 chickens gathered in 31 farms were used in the experiment. Characteristic for Mareks disease (MD) lesions were observed in 126 examined birds (49.2%). The viral antigen in feather follicles was detected in 145 chickens (56.6%). The results of necropsy showed 80.8% accordance with RID test. The organ sections were the source of total cellular DNA. Primers specific for 132 bp sequence of MDV genome and for viral gene meq were used for two different PCRs. The obtained results were identical in both molecular reactions. The presence of the viral DNA was confirmed in 181 samples (70.7%). The accordance of RID test and PCR results was valued at 85.9%. The percentage of positive results in RID was valued at 80.1% positive results for PCR. The specificity of PCR were 100%. The conducted experiment confirmed that the PCR is a valuable and efficient supplementary method of the classical diagnostic techniques of MD. |
| Keywords: Mareks disease, RID, gen meq, 132 bp sequence, PCR |