Medycyna Wet. 66 (2), 106-108, 2010
full text
| Niedbalski W.. |
| Comparison of four ELISAs for the detection of antibodies against bluetongue virus |
| The aim of this study was to determine the diagnostic specificity, sensitivity and repeatability of four commercially available ELISA kits for the detection of antibodies against bluetongue (BT) virus. The relative specificity of ELISAs was estimated using a panel of sera originated from healthy cattle never vaccinated nor exposed to BT virus. All ELISA kits had a high relative specificity (99.2-99.4%). The relative sensitivity of ELISAs estimated using a panel of sera collected from BTV infected cattle was also high and similar for all kits (97.4-100%). However, the relative sensitivity evaluated on the basis of testing of vaccinated animals was different for the used ELISAs: the LSI, ID VET and Ingenasa kits had a high sensitivity (85.2-98.2%) but the sensitivity of VMRD ELISA was much lower (68.6%). The repeatability of ELISAs was expressed as a coefficient of variation (CV) of results of sera tested 5 times in the same day and 10 times in different days through the period of 2 months, by the same person, in the same conditions, and by using of the same equipment. The CVs of sera tested in Ingenasa and ID VET kits ranged from 6.1 to 9.8% and were below the 10% threshold adopted as a maximal for the acceptable repeatability of a method. In conclusion, it can be stated that the applied ELISAs can be a valuable diagnostic tool for the serological monitoring studies in the BTV infected premises. Nevertheless, the Ingenasa and ID VET ELISAs can be the most useful in sero- -surveillance of livestock following vaccination. |
| Keywords: bluetongue, serology, ELISA kits, comparison |