Med. Weter. 68 (5), 290-292, 2012

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Strachecka A., Borsuk G., Paleolog J., Chobotow J.
Anti-pathogenic activity on the body surface of adult workers of Apis mellifera
The aim of the study was to determine the anti-pathogenic activity on the body-surface of bee workers after applying selected acaricides, such as Amitraz or oxalic and formic acids. In two consecutive years, four experimental groups (5 colonies per group) were created. The 1st group was the control, the 2nd was treated with Apiwarol, the 3rd with oxalic acid, and the 4th with formic acid. Hive worker bees were collected three times a year from each colony: just before treatment as well as two and four weeks after treatment. The samples were shaken/rinsed for 10 min. at 8000 rpm, filtered and lyophilized. Anti-pathogenic activities were determined by the double application method, with SABG (Aspergillus niger, A. fumigatus), YPD (Candida albicans) and LB (Staphylococcus ureus, Bacillus subtilis, Micrococcus luteus, Salmonella Typhimurium, Pseudomonas aeruginosa and Escherichia coli). The control group displayed antifungal and antibacterial activities against all of the pathogens analysed. After acaricide treatment, anti-pathogenic activities were reduced or disappeared within the four weeks of the experiment.
Keywords: acaricides, honey bee, anti-pathogenic activity, bacteria, fungus