Med. Weter. 74 (6), 401-404, 2018
full text
| DANUTA WROŃSKA, BOGDAN FELIKS KANIA, SYLWIA PAŁKA, KONRAD KOZIOŁ, MICHAŁ KMIECIK |
| Glutamate influence on catecholamine release from rabbits hypothalamus – ex vivo |
| The aim of the study was to find the direct effects of possible correlation between different of the glutamate concentrations (5, 50 and 200 µM) from the one hand and catecholamine release from rabbit hypothalamus homogenate during 90 min incubation from the other hand. Brain structure were obtained from decapitated female 12 week old rabbit. After decapitation brain of animal obtained was placed in 0.9% NaCl. Then, each brain structure from each rabbit (approx. 50 mg) was placed in incubation wells (cell culture Sigma) containing 1 ml of incubation medium (Krebs phosphate buffer containing glucose 0.3% bovine serum albumin and 0.1% BSA) without (control) or three doses of Glu in a concentration of: I – 5 μM, II - 50 μM and III - 200 μM. Each slice of tissue postponed scraps of each brain structure every 30 minutes to the next holes. Medium collected after 30, 60 and 90 minutes of experiment were used for dopamine, noradrenaline and adrenaline RIA measurement. The obtained results show that Glu influences directly on catecholamines release from hypothalamus as an central brain structure of the HPA axis in stress inducing and adaptive processes. Generally, there was an inhibitory effect on catecholamines release from hypothalamus homogenates. Our findings show that Glu more influences the brain's motivational structure, which may indicate its contribution to the stress response by modulating the amount of catecholamine released.. |
| Key words: hypothalamus, glutamate, catecholamines, rabbit |