Med. Weter. 80 (7), 330-335, 2024
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| MARTA KUCHTA-GŁADYSZ, ANNA GRZESIAKOWSKA, JOANNA WOJCIECHOWSKA-PUCHAŁKA, BARBARAKIJ-MITKA, MARCIN PRZYBYŁO, OLGA SZELESZCZUK PAWEŁ GÓRKA, MONIKA BUGNO-PONIEWIERSKA |
| Preliminary research to determine spontaneous instability in the Reeves’s muntjac genome |
| Reeves’s muntjac is one of the species of the Muntjac deer taxonomic group of Ruminantia which has a high karyotype variability in terms of structure, but above all in the number of chromosomes. The aim of the study was to determine the level of instability of cellular nucleus chromatin in this species using cytogenetic tests: comet assay (SCGE), sister chromatid exchange assay (SCE), micronucleus assay (MN) and fragile site assay (FS). The study was performed on a group of 7 individuals (Reeves’s muntjac). All animals were 2-5 years of age and were in a good health condition. The study material consisted of peripheral whole blood lymphocytes (2.5 mL each animal). The analysis exhibited considerable diversification between levels of spontaneous damage to chromosomes and DNA in individual tests. The largest differences in the level of chromosome instability among individuals of Reeves’s muntjac were noted in the comet test. The mean percentage of DNA in comet tail was 3.31 ± 0.43%. The comet test is the only method among the applied techniques of genome instability assessment which allows for exact determination of the degree of breaks directly in DNA strands. Other three tests used (SCE, CBMN and FS) also enable identification of damage to DNA strands but at a higher level of its organisation in the genome. |
| KEY WORDS: Reeves’s muntjac, spontaneous chromosome instability, DNA integrity, biomonitoring |