Med. Weter. 81 (1), 23-31, 2025
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| RÉDHA BELALA, SEDDIK KEBBAL, CHAOUKI BOUGUETOF , MYRA MEDJKOUNE, CHOAYB MECHEROUK, NORA MIMOUNE |
| Morphometric parameters of canine spermatozoa: Comparison between conventional microscopy and CASA system |
| The present study aimed to evaluate the effect of two cell detection parameters (Head Brightness Minimum „HBM” and head surface „AREA”) on the automatic morphometric analysis of canine spermatozoa (spz) by using Hamilton-Thorne (HT)-IVOS II CASA system. Twenty ejaculates were collected from 6 identified dogs and the spz morphology was analyzed by conventional technique (microscopy) and by the HT-IVOS II system in two different situations (mobile and static spz). The 20 videos recorded from the preliminary analysis of the ejaculates were reassessed by modifying the two parameters of the setup studied according to an optimization protocol which made it possible to obtain 1120 IVOS II analyses. Statistical analysis was performed using IBM-SPSS software. Data showed a very significant effect of HBM on normozoospermia and teratozoospermia rates. On the contrary, the AREA factor did not affect spz quality except for the extreme values (14-16 μm²). Indeed, the HBM of 140 and the AREA between 4-10 μm² seemed to be the most suitable cell detection setting for automatic morphometry by the HT-IVOS II system and it can constitute an alternative to microscopy. In addition, Improper parameterization of the HBM could induce contrast artifacts leading to an overestimation of the major anomaly “Proximal Droplet”. SPZ immobilizing before analysis by the HT-IVOS II system did not improve the results. To conclude, the authors suggest that manufacturers of CASA systems should ensure complete validation of the settings loaded on their systems in order to prevent random adjustments by users leading to great intra- and inter-laboratory variability in the results of automatic morphometry. |
| Keywords: dog, semen, CASA, light microscopy, automatic morphometry. |