Med. Weter. 81 (6), 284-287, 2025
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| LINQUAN WANG,CHENGJIEPAN |
| Mir-208 inhibits cardiomyocyte apoptosis in acute myocardial infarction in rats by targeting GATA4 to activate qk15 related signaling pathway |
| Objective: The objective of this study was to investigate the potential role of mir-208 in blocking myocardial cell apoptosis during acute myocardial infarction (AMI) via activating the qk15-related signal pathway through targeting GATA4. Methods: Three groups of forty-five clean male Wistar rats were assigned at random: sham operated group (open chest only, without ligation of left anterior descending coronary artery), model group and low expression group (tail vein injection of mir-208 inhibitor after model establishment). Each group had 15 rats. The changes of myocardial function (MI) were measured by ECG. The changes of LDH in serum were measured by colorimetry. The infarct area was calculated. TUNEL method was used to detect cardiomyocyte apoptosis. Western blot was used to assess the expression levels of qk15, GATA binding protein 4, and caspase-3. Results: In contrast to the sham group, the expression level of mir-208, serum LDH level, MI area, apoptosis rate of myocardial cells and caspase-3 expression levels of rats in the model group were increased, and the expression levels of EF, SV, FS, GATA4 and QK15 were decreased. In contrast to the model group, the expression level of mir-208, serum LDH level, MI area, myocardial cell apoptosis rate and caspase-3 expression levels of rats in the mir-208 low-expression group were reduced, while the expression levels of EF, SV, FS, GATA4 and QK15 were significantly increased. Conclusion: Down regulation of mir-208 expression can inhibit apoptosis of myocardial cells in AMI, which may be achieved by targeting GATA4 to activate qki5 related signaling pathway. |
| Keywords:Mir-208, GATA4, qk15 related signaling pathways, AMI, cardiomyocyte apoptosis |