Medycyna Wet. 64 (5), 677-680, 2008
| Skierka E., Sadowska M., Mańkowska J., Lipińska-Beyer A. |
| Enzymatic hydrolysis of protein of Baltic cod backbones |
| Enzymatic hydrolysis was conducted to recover the potentially addible high protein hydrolysate from cod backbones. The commercially available alcalase and trypsyne was used for hydrolysis at enzyme concentrations of 2.5; 5; 10; 15; 20; 30 and 40 mg/g backbones. The enzymatic deproteinization was conducted for 24 and 48 h. All procedures were performed at pH 7.0 and 4C. The yield of enzymatic hydrolysis increases with the growth of trypsine and alcalase concentration up to 20 and 10 mg/g backbone, respectively. The increase of these values did not influence the yield of enzymatic deproteinization. After the treatment by trypsyne, maximum recovery of protein from cod backbone was 60 %, while by alcalase it was 55 %. The yield of enzymatic hydrolysis was the same after 24 and 48 h. Collagen losses did not exceed 0.2 %. |
| Keywords: fish, protein, by-products, backbone |