Med. Weter. 82 (1), 14-20, 2026
full text
| JIAYU CUI, YUNJIAO CHEN, DAWEI WANG, ZHILIN LIU, XIN ZHANG, ZELIN JIA, XUELI WANG |
| Establishment and application of the colloidal gold test strip method for the detection of pathogenic Bacillus cereus of bovine origin |
| Bacillus cereus is a Gram-positive rod-shaped bacterium widely distributed in natural environments. Strains of this opportunistic pathogen can cause a range of illnesses, including bacteremia, food poisoning, localized infections, and severe systemic diseases, leading to substantial economic losses and adverse effects on related processing industries and livestock farming. Consequently, the establishment of a rapid detection method for Bacillus cereus is urgently needed to facilitate timely and effective prevention and control of this pathogen. In this study, polyclonal antibodies and colloidal gold solutions were prepared, followed by optimization of spray concentrations for the control line (C line) and test line (T line). Various types of nitrocellulose (NC) membranes were also evaluated. After assembly, the test strips were systematically assessed. The results indicate that the optimal detection was achieved when the antigen was labeled at 10 μg/mL, with the C line sprayed at 1 mg/mL and the T line at 0.5 mg/mL. Sartorius CN140 was identified as the most suitable NC membrane. The colloidal gold test strip showed no cross-reactivity with Bacillus thuringiensis, Bacillus mycoides, Bacillus subtilis, Bacillus licheniformis, Staphylococcus aureus, or Escherichia coli. Validation experiments confirmed that the test strips exhibited high sensitivity, specificity, and stability. When challenged with closely related species and other genera, the immunochromatographic strip demonstrated excellent specificity and sensitivity, offering a reliable tool for rapid clinical detection of Bacillus cereus infections. |
| Keywords: Bacillus cereus; colloidal gold test strips; application |